ISOLATION AND CHARACTERISATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT (CASE STUDY OF ENUGU)


ISOLATION AND CHARACTERISATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT (CASE STUDY OF ENUGU)  

ABSTRACT 

This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, which were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile pipette and test tubes. The dilution were  then cultured on different  media, macconkey agar, nutrient agar and blood agar end later incubated at 370c for 24 hours. Discrete bacteria colonies were observed and each colomy was gram stained and examined microscopically. Biochemical test were carried out to confirm the particular bacteria present. Bactering isolated  were Staphylococcus aureus, Bacillus, lactobacillus, streptococcus and Escherichia coli. Most at this   microorganisms isolated are pathogenic and are toxic when ingested in contaminated suya meat. They are capable of causing illness. For instance  staphylococcus aureus produce air  exoticin and can withstand temperature of 1000c for 30 minutes. When ingested, if cause intoxication manifesting in 4-5 hours with acute diarrhoes vomiting and gastroenteritis lasting 6-8 hours. 

 

TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of contents 

CHAPTER ONE 

INTRODUCTION

Aims and objective

Hypothesis

Statement of problem

Significance

 Limitation

CHAPTER TWO 

Literature review

CHAPTER THREE 

Materials

Reagents

Media used

Sugar used for biochemical test

Preparation of media

Collection of samples

Method used

Pour plate method

Staining technique used

Biochemical test used

Coagulates test

Motility test

Indole test

Sugar fermentation test

CHAPTER FOUR 

Result of the analysis

Identification of the isolate

Confirmatory result

CHAPTER FIVE 

Discussion and conclusion

Recommendation

References

CHAPTER ONE

INTRODUCTION

Meat is an animal product. It is that part of animal that provides major source of protein in west African food. 

There are different types of meat from different types of animals, eg pork meat (pig) multon (goat) beef (cow).

          Meat can be served as prepared meat product eg corn beef, fried meat, cooked meat and suya, meat (smoke meat). Meat is a perishable food and its composition is ideal for the growth of wide range of spoilage bacteria. Public concern has vision due to numerous food scandals such as those surrounding bovine spongiform encephalopathy and roof and mouth disease epidemics and food borne disease which remain substantial burden . We can meet those challenges with an improved and global food safety control system. One possible improvement would be a rapid and accurate detection system for microbial spoilage. This technique should ideally also be non destructive and give result in real time for application in highly automated food  processing environment.

Suya meat is produced by smoking the raw beef with the addition of some spices, salt, oil, groundnut cake and flavors. The meat is first sliced into smaller pieces and the spices are rubbed onto it, it is later oven dried or over a local source of heart. This allows the meat to get dried properly with the right taste before it is sold to the consumers. 

This is sold at a specific joint or hawked. when it is sold at joint it is constantly kept warm over fire source. The hawked saya meat is carried about in open basin from place to place thereby exposing it to  dust and other effects of the  environment .by doing so, harmful organisms find their ways into the meat there by causing poisoning. 

Food poisoning is an illness with acute gastro enteritis. it is as a major symptom caused by the ingestion of food causing  harmful micro organisims or harmful substances. Tomatari (1983).

Some of the micro organisms present in suya that causes food poisoning when consumed  are :  Salmonella typhi,staphylococcus aureus,  clostridum butilinum,  clostridium  preferring Bacilluscerecesstreptococcusprogenies. 

 Some of these micro organisms in meat cause off shavours which make the meat to be unfit for eating and also reduce the taste value. 

AIMS OF OBJECTIVE 

This project research is designed to isolate and identify the common spoilage organisms of suya meat often production thereby ensuring the safety of the population at risk in eating containineted suya meat 

HYPOTHESIS 

HO: Hawked suya meat sold around ESBS metropolises contain pathogenic microorganisms 

H1 Hawked suya meat sold around KSBS metropolies does not cantinas pathogenic micro organisms.

HI Hawked suya meat sold around KSBS, metropolies contain micro organisms but the load is not enough to cause disease. 

SIGNIFICANCE OF STUDY 

The significance of this work is to enable those producers to improve the hygiene of the food (suya meat) and a good knowledge of safe food handling practice, suitable protective clothing to be worn  and it will also enable us to be aware of various pathogenic organic and infection they  transmit through food (suya meat) . As a result of this, the prevention of contamination of food is to be adhered to .This can be achieved by

-keeping high risk food at temperature that will inhibits the growth of bacteria  

⦁ Ensuring that during preparation, food is in the danger zone should be eliminated as fast as possible.

⦁ Using suitable preservation such as salt and sugar.

⦁ Using various packing method like glass wares. 

STATEMENT OF PROBLEM 

Pathogenic organisms are organization that are referred to as specific health hazards associated with gastro intestinal disturbance causing  illness. The victim suffered from obdominal pain and diarrhea with more vomiting thus diarrhea usually manifest the illness, which when untreated, may result to death. This has now made interest on how we can improve on our food hygiene to avoid contamination. 

LIMITAION 

The project work is limited to the isolation and identification of bacteria associated with hawked Suya meat sold around KSBS  Kastina state. This will help in increasing the health awareness of the public on the damages of eating hawked Suya meat. The project work would have covered the whole of Kastina urban but it was not possible due to financial  and time constraints. During the course of this project work, constant power failure was experienced and this possessed a problem to media preparation and incubation. 

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ISOLATION AND CHARACTERISATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT (CASE STUDY OF ENUGU)



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