PROXIMATE AND PHYSICOCHEMICAL ANALYSIS OF FISH FLOUR OBTAINED FROM THREE FISH SPECIES
Work on production proximate and physicochemical analyses of fish flour produced from Tilapia, cat and stock fishes was investigated. The fish species after harvesting were processed hygienically to flour. Proximate analysis result shows that catfish has high moisture content of 9.79, followed by Tilapia fish which has 9.5% and stock 8.8% protein content is high in stockfish 69.9%, then catfish 68.0% and tilapia fish which have 65.7%, and the carbohydrate level in stock fish is 7%, 5.3% in Tilapia fish and 4.7% in cat fish. Then the physicochemical analysis gives the total volatile bases of stock fish as 5.6%, followed by Tilapia fish 3.5% and 2.8% catfish. Organoleptic analysis result showed that there was no significant difference among the samples in terms of colour, taste, aroma, texture and overall acceptability. Acceptable fish flour could therefore be produced from fish species.
TABLE OF CONTENT Title page i Certification ii Dedication iii Acknowledgement iv Table of content v
CHAPTER ONE 1.0 Introduction 1 – 2 1.1 Fish protein concentrate 2 – 3 CHAPTER TWO 2.1 Literature Review 4 2.2 Different techniques of fish processing and preservation these include 4 2.3 Tilapia 5 – 6 2.4 Catfish 6 – 7 2.5 Stockfish 7 – 9 2.6 Fish nutrient 9 2.7 Fish product 10 2.8 Nutritional significance 10 – 11 CHAPTER THREE 3.0 Materials and Method 12 3.1 Materials 12 3.2 Method 12 3.2.1 Production of fish 12 – 13 3.3 Proximate analysis of fish flour 13 3.3.1 Mositure content determination 13 3.3.2 Protein determination 14 3.4 Mineral analysis 15 3.5 Organolptic analysis 15
CHAPTER FOUR 4.0 Results and discussion 16 4.1 Results on proximate analysis 16 4.2 Proximate analysis result table 16 – 18 4.3 Mineral analysis result 18 – 19 4.5 Organoleptic analysis results 20
CHAPTER FIVE 5.0 Conclusion and recommendation 21 5.1 Conclusion 21 5.2 Recommendation 21 References 22 Appendix 23 – 25
1.0 INTRODUCTION Food is a biological material which has aesthetic appeal, good organoleptic qualities, which when ingested, digested and absorbed by the body will supply the needed nutrients for growth, maintenance of health and support of other metabolic activities within the body system. There are two major sources of food as a biological material, these include food from plant and animal sources food for plant sources include cereal, roots, tuber, pulses etc. while those from animal sources include meat, milk, eggs, fish etc. Food commodities of animal origin are of utmost importance in diets due to the presence of a variety of essential nutrients. The essential nutrient which this group of food commodities are noted is protein, which is the only nutrient that takes pant in all the functions which nutrients perform in the body. Even though plant food commodities like cereals and legumes, have appreciable amounts of protein in varying quantities, such proteins are of lower quality compared to that of animal sources due to the deficiency of plant protein in certain essential amino acids like lysinc, methionine and cysterine. Methionine and cysterine on the other hand, animal protein has a full complement of all essential amino acids, food play a major role in our physical and mental well-being and it is needed to nourish the body and also plays a significant role in our social life protein from animal origin are referred to as complete proteins. Fish is an aquatic animal used as human food after processing. All true fish muscles is typical striated like other animal foods is a good source of protein of high biological value. However, unlike that of meat, the protein in fish contains small amount of connective tissues (which bind the muscle fibres together), but contains no elastin (the material the wall of the fibres is made of) this makes the cooking of fish muscle faster, and the cooked fish muscle more easily digested compared to that of meat. Fish varieties that are high in fat, e.g salmon, tuna and herring supply considerable amounts of energy from the fat, which is dispersed throughout the fish, flesh, and therefore normally invisible, unlike that fat deposited in meat carcass. Also, over 70% of protein comes from animal sources in developed countries. Generally animals fats contain more percentage of saturated fatty acids while vegetables of plants oils have a high proportion of unsaturated fatty acids. Protein from animal soruces are of better quality than those from plant sources, since they are complete proteins while vegetable proteins are incomplete proteins in that they are deficient in one or more essential amino acids. Fish are good suppliers of phosphorus and iron, but very little calcium, unless the bones are eaten. The fatty fish are also valuable sources of fat soluble vitamins, especially A and D. the cod liver is particularly high in these soluble vitamins. (Adepeju, 2010).
1.1 Fish Protein Concentrate Fish protein concentrate (FPC) is any stable fish preparation intended for human consumption in which the protein is more concentrated than in the original fish. It is accepted as human food and not animal food while fish meal is not accepted as human food because of its comparatively poor flavor stability in general requiring antioxidants for flavor maintenance, its odor and also the fact that many countries will not permit the sale of foods made from unwholesome raw materials e.g fish guts. One way of supplementing the nutritional deficiency of a cereal diet lies in eating fish which supply high quality protein. Fish protein concentrate resolves the high problem in finding an inexpensive way of nutritional supplement. Fish protein concentrate is a good source of protein because it contains about 80 percent protein and the protein quality is high presenting the amino acid in the right balance for human nutrition. Fish protein concentrate is an odorless, tasteless powder which is relatively unattractive to eat by itself, it has to be incorporated into other foods such as bread, biscuit, soaps and stews at a level that does not affect their normal properties. Good results have been obtained with macaroni products, a milk shake drink, spaghetti sauce, infant foods, dietetic food and breakfast cereals. Fish just like any other food products or agricultural product is liable to deterioration and spoilage. Hence, means of preservation and processing must be sought in order to alleviate these problems. One way of averting these problems is through drying processes and milling into fine flour in order to elongate the shelf life of the product. This project work is therefore on the production of fish protein flour from three species of fish: Tilapia, cat fish and stock fish. Therefore the objectives include:
Production of fish protein flour from three fish species: Tilapia fish, cat fish and stock fish Proximate analysis of the samples mineral analysis of the samples Organoleptic analysis of the samples